FLOWFacility

Flow cytometry facility and service

Flow cytometry is a powerful and versatile technology allowing to assess up to 50 parameters (either physical, biological or molecular) on thousands of particles per second.

This makes flow cytometry the technology of choice for a wide range of applications, including cell counting, immunophenotyping, detection of fluorescent proteins, cell viability, apoptosis and proliferation, cytokines detection and measurement, cell cycle and DNA content analysis, telomere length measurement, calcium flux.

Moreover, it is possible to exploit the measured parameters to physically separate specific cell subpopulations of interest based of physical and biological characteristics.

Our facility is equipped by two cell analyzers and one cell sorter .

For additional information FEEL FREE TO contact us

flowfacility.biometra@unimi.it

Instruments

  • Equipped with 5 lasers:

    • UltraViolet 355nm

    • Violet 405nm

    • Blue 488nm

    • Yellow-Green 561nm

    • Red 633nm

    Equipped spectral technology.

    While conventional flow cytometers assign each of their fluorescence detectors to measure unique fluorochromes around their emission maximum, spectral flow cytometers detect the entire light emission spectrum for every fluorescent molecule, allowing thus to measure up to 50 parameters (2 physical parameters and 48 fluorescence) at the same time at single cell level.

  • Equipped with 4 lasers:

    • Violet 405nm

    • Blue 488nm

    • Yellow-Green 561nm

    • Red 638nm

    Equipped with advanced Avalanche Photodiodes providing high-efficiency, low-noise light detection.

    Capable to detects up to 15 total parameters (2 physical parameters and 13 fluorescences) and to nanoparticles down to 80 nm.

  • Equipped with 4 lasers:

    • Violet 405nm

    • Blue 488nm

    • Yellow-Green 561nm

    • Red 638nm

    Capable to measure up to 17 parameters (2 physical parameters and 15 fluorescence) on single cells and sort up to 4 different cell populations.

    Different collection modes are available, from 15-mL tubes to 1.5-mL microtubes, culture plates and slides.

services provided

  • Performed by our trained specialists for both internal (University of Milan, Department of BIOMETRA) and external users.

    The facility staff will provide information on sample preparation at the time of the experiment planning.

    • 80€/h for internal users with technician

    • 150€/h for external users with technician

  • Performed by our trained specialists for both internal and external users.

    The facility staff will provide information on sample preparation at the time of the experiment planning.

    Conventional mode:

    • 50€/h for internal users with technician

    • 70€/h for external users with technician

    Spectral mode:

    • 60€/h for internal users with technician

    • 80€/h for external users with technician

  • Allowed only to internal users after a training period with a final practical exam.

    Conventional mode:

    • 40€/h for internal authorised users

    Spectral mode:

    • 50€/h for internal authorised users

  • Basic theoretical training, and instrument use training courses are offered to internal users on request.